GO:0006296:   nucleotide-excision repair, DNA incision, 5"-to lesion
The endonucleolytic cleavage of the damaged strand of DNA 5" to the site of damage. The incision occurs at the junction of single-stranded DNA and double-stranded DNA that is formed when the DNA duplex is unwound. The incision follows the incision formed 3" to the site of damage.