GO:0006428:   isoleucyl-tRNA aminoacylation
The process of coupling isoleucine to isoleucyl-tRNA, catalyzed by isoleucyl-tRNA synthetase. The isoleucyl-tRNA synthetase is a class-I synthetase. The activated amino acid is transferred to the 2"-OH group of a isoleucine-accetping tRNA. The 2"-O-aminoacyl-tRNA will ultimately migrate to the 3" position via transesterification.